Bimolecular Affinity Purification (BAP): Tandem Affinity Purification Using Two Protein Baits
نویسندگان
چکیده
منابع مشابه
Identification of Protein Interacting Partners Using Tandem Affinity Purification
A critical and often limiting step in understanding the function of host and viral proteins is the identification of interacting cellular or viral protein partners. There are many approaches that allow the identification of interacting partners, including the yeast two hybrid system, as well as pull down assays using recombinant proteins and immunoprecipitation of endogenous proteins followed b...
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With the number of complete genome sequences increasing at an ever-faster pace, we are getting sequence data for the full complement of proteins or “proteome” (Wasinger et al. 1995; Wilkins et al. 1996), encoded by various organisms. To understand how the proteome confers specific properties to a host cell, one needs to obtain information regarding several parameters, including the level of exp...
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متن کاملProtein Purification by Affinity Chromatography
The preparation of a number of agarose and polyacrylamide bead derivatives useful in the purification of proteins by afhnity chromatography is described. These techniques permit (a) the attachment of ligands to the gel through extended hydrocarbon chains which place the ligand at varying distances from the gel matrix backbone; (b) the covalent attachment of ligands to agarose or polyacrylamide ...
متن کاملProtein Purification by Affinity Chromatography
The preparation of a number of agarose and polyacrylamide bead derivatives useful in the purification of proteins by afhnity chromatography is described. These techniques permit (a) the attachment of ligands to the gel through extended hydrocarbon chains which place the ligand at varying distances from the gel matrix backbone; (b) the covalent attachment of ligands to agarose or polyacrylamide ...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2009
ISSN: 1559-6095
DOI: 10.1101/pdb.prot5318